Pyrophosphorolytic dismutation of oligodeoxy-nucleotides by terminal deoxynucleotidyltransferase
نویسندگان
چکیده
منابع مشابه
Pyrophosphorolytic dismutation of oligodeoxy-nucleotides by terminal deoxynucleotidyltransferase.
Terminal transferase (TdT), when incubated with a purified(32)P-5"-end-labeled oligonucleotide of defined length in the presence of Co(2+), Mn(2+)or Mg(2+)and 2-mercaptoethanol in cacodylate or HEPES buffer, pH 7.2, exhibits the ability to remove a 3"-nucleotide from one oligonucleotide and add it to the 3"-end of another. When analyzed by urea-PAGE, this activity is observed as a disproportion...
متن کاملBiosynthesis of murine terminal deoxynucleotidyltransferase.
An immunoprecipitation assay for measuring synthesis of murine terminal deoxynucleotidyltransferase (EC 2.7.7.31) has been developed using rabbit antiserum to calf terminal transferase. The antiserum precipitates a single Mr = 60,000 polypeptide (TdT-60) from all cell lines and tissues that contain enzymologically demonstrable terminal transferase. This polypeptide is not precipitated from labe...
متن کاملUbiquitylation of Terminal Deoxynucleotidyltransferase Inhibits Its Activity
Terminal deoxynucleotidyltransferase (TdT), which template-independently synthesizes DNA during V(D)J recombination in lymphoid cells, is ubiquitylated by a BPOZ-2/Cul3 complex, as the ubiquitin ligase, and then degraded by the 26 S proteasome. We show here that TdT is ubiquitylated by the Cul3-based ubiquitylation system in vitro. Because TdT could also be ubiquitylated in the absence of Cul/B...
متن کاملTerminal deoxynucleotidyltransferase. Serological studies and radioimmunoassay.
Mouse antisera against calf terminal deoxynucleotidyltransferase (terminal transferase) have been prepared. The sera have been used to characterize terminal transferase both by studying inhibition of enzyme activity and by developing a competition radioimmunoassay using highly purified 125I-labeled terminal transferase. By either assay, anti-terminal transferase serum did not cross-react signif...
متن کاملBiochemical properties of purified human terminal deoxynucleotidyltransferase.
The kinetic mechanism and limited substrate specificity of purified terminal deoxynucleotidyltransferase from human leukemic cells is described. The reaction which utilizes two substrates, dATP as monomer, and p(dA), as initiator (where n is the chain length from 4 through 50), proceeds by a rapid equilibrium random mechanism. The polymerization reaction occurs only in the presence of a divalen...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Nucleic Acids Research
سال: 1999
ISSN: 0305-1048,1362-4962
DOI: 10.1093/nar/27.15.3190